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. 2009 Dec 18;285(10):7111–7118. doi: 10.1074/jbc.M109.052977

FIGURE 5.

FIGURE 5.

Increased proportion of slow fibers in APOBEC2−/− mouse soleus. A, immunohistochemical staining with an anti-slow myosin HC (MyHC) mAb of transverse sections of soleus muscle from 15-week-old wild-type (WT) and APOBEC2−/− (A2−/−) mice. Analysis of five pairs of 15-week-old mice revealed that the proportion of soleus fibers staining with the anti-slow myosin HC mAb increased from 31% in control mice to 40% in APOBEC2-deficient mice. B, comparison of the relative abundance of slow and fast myosin HC isoforms in soleus muscle (SL; left and middle panels) and tibialis anterior muscle (TA; right panel) from 3-month-old litter-matched wild-type, APOBEC2+/−, and APOBEC2−/− mice analyzed by silver staining of muscle extracts subjected to SDS-PAGE in low cross-linked gels. C, histogram showing the ratios of fast (MyHCIIa+IIx) to slow (MyHCI) myosins in soleus muscle of seven wild-type and nine APOBEC2-deficient mice as judged by densitometry of silver-stained SDS-polyacrylamide gels.