FIGURE 5.

Altered expression of cold-responsive genes in crlk1 knock-out mutants and complementation lines. A, Northern analysis showing the altered expression of CBF in wild-type and crk1 mutant plants after cold treatment. The plants were treated at 4 °C for 0–2 h. 18 S RNA shows equal loading. B, Northern analysis showing expression of RD29A, KIN1, and COR15a genes in wild-type, crlk1 mutant plants, crlk1/CRLK1 (complementation lines with full-length CRLK1) and crlk1/ΔC2 (complementation lines with truncated CRLK1 lacking the C-terminal CaM-binding region) under cold treatment (4 °C) for various periods. 18 S RNA shows equal loading of total RNA. Ten μg of total RNA was loaded in each lane. The DNA fragments amplified with gene-specific primers were used as probes.