FIGURE 6.

Hierarchical clustering of Golgi membrane and associated proteins that showed significant changes upon treatment with mitotic cytosol. Hierarchical clustering using Pearson correlation was performed on the proteins in supplemental Table 3 to generate the heat map. As an overview, part of the clustering result is shown in A. Two representative clusters showed unique patterns were selected and the averaged fold changes (MC and MC → IC versus IC) from three separate experiments were plotted for each protein (B and C). In contrast to the proteins showed significant changes, many Golgi structural proteins showed little change. Several such proteins were selected as representatives for a very distinct cluster, and their average ratios under the three indicated conditions were also plotted (D).