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. 2010 Jan 6;285(10):7334–7343. doi: 10.1074/jbc.M109.061051

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — αS oligomers do not associate with inhibited complex I activity. The brains of a cohort of 12 A53T αS tg mice 8–9 months old (Symptomatic and asymptomatic) were divided in hemispheres. a, αS soluble oligomers in high speed cytosols (15 μg protein) of A53T αS tg mice. The samples were treated at 65 °C overnight prior to gel loading and blotting with LB509 antibody. b, densitometry of Western blot in a, presented as the ratio of αS oligomers (migrating higher than 35 kDa)/monomer (migrating as 17 kDa) and normalized to actin. c, complex I activity measured in purified mitochondria from the respective mouse brain hemisphere. The activity of complex I (rotenone-sensitive NADH:CoQ oxidoreductase) was measured as described for b. d, no correlation between human A53T αS oligomerization and complex I activity (n = 12). e, no correlation between endogenous mouse αS and complex I activity (n = 9).