FIGURE 5.

Molecular communication between the N and the C termini. Plasma membrane-enriched fractions of yeast strains expressing the various mutants of the PM H⁺-ATPase were run on SDS-PAGE and transferred to nitrocellulose for immunostaining or overlay assay. A, N-terminal truncated mutants of the PM H⁺-ATPase show increased level of phosphorylation and bind more 14-3-3 proteins than the wild type. pThr, phosphothreonine. B, the high capacity for 14-3-3 binding of the ΔN10aha2 PM H⁺-ATPase is strictly dependent on the penultimate Thr-947 residue.