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Activation of the PM H+-ATPase by N-terminal truncations is dependent on Thr-947 phosphorylation but can be surpassed by a modification of autoinhibitory region I. A, a yeast complementation test of aha2 T947A and N-terminal truncated mutants hereof. B, a yeast complementation test of aha2 G867A,T947A and N-terminal truncated mutants hereof. All yeast cells were spotted in optical densities (OD) of 0.1 and 0.01 on solid glucose-containing medium with different pH (5.5 and 4.5).
