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. 2010 Jan 12;285(10):7344–7350. doi: 10.1074/jbc.M109.096123

FIGURE 7.

FIGURE 7.

Mutagenesis of the conserved Glu-10 residue in the N terminus of the PM H+-ATPase perturbs autoinhibition. RS-72 transformed with the indicated mutants was spotted on plates with different pH (5.5, 4.5, and 3.5) and in two different optical densities (OD), namely ODs of 0.1 and 0.01. Gly-867 resides in autoinhibitory region I, and Arg-913 resides in autoinhibitory region II (see Fig. 1A).