FIGURE 5.

rLOX-PP inhibits FGF-2 binding to MC3T3-E1 osteoblast cell layers. Preconfluent MC3T3-E1 cells were grown in 24-well plates (2.5 × 10⁴ cells/well) in medium containing 0.1% BSA for 24 h. An equilibrium binding assay was then performed as described under “Experimental Procedures” with ¹²⁵I-FGF-2 in the presence or absence of increasing concentrations of rLOX-PP (0–8 μg/ml). Wells were then washed, and bound ¹²⁵I-FGF-2 was measured. Specific binding was assessed using 500-fold excess nonlabeled FGF-2. Data are expressed as percent of FGF-2 bound/well measured in the absence of rLOX-PP. Data from three separate experiments were pooled (n = 10, *, p < 0.05).