TABLE 1.
Peptides detected from tryptic digests of acetylated band detected in mammalian mitochondrial ribosomal proteins by LC-MS/MS analysis
Collision-induced dissociation spectra of peptides were searched by MASCOT as described in the supplemental materials, and the spectra for each peptide are provided in supplemental Table 1. Modifications of peptides by acetylation (ac), phosphorylation (p) and oxidation (o) are shown for each peptide.
| Peptide sequence | m/z | Mr (experimental) | Score |
|---|---|---|---|
| MRPL10 | |||
| QKLoMAVTEYIAPKPVVNPR | 725.1 | 2172.3 | 39 |
| QacKLoMAVTEYIAPKPVVNPR | 738.8 | 2213.3 | 22 |
| LoMAVTEYIAPKPVVNPR | 958.5 | 1915.1 | 72 |
| oMIAVCQNVAoMSAEDK | 822.5 | 1642.9 | 96 |
| VFPNQILKPFLEDSK | 888.4 | 1774.8 | 69 |
| YQNLLPLFVGHNLLLVSEEPK | 1213.1 | 2424.1 | 96 |
| VKEoMVRILK | 567.7 | 1132.3 | 21 |
| VacKEoMVRILK | 588.5 | 1173.5 | 15 |
| ILacKpSVPFLPLLGGCIDDTILSR | 858.0 | 2571.0 | 47 |
| QGFINYpSacKLPpSLALAQGELVGGLpTLLTAR | 1104.6 | 3310.9 | 54 |
| LPSLALAQGELVGGLTLLTAR | 1048.2 | 2094.3 | 145 |
| THSLLQHHPLQLTALLDQYAR | 1229.3 | 2455.8 | 78 |
| QQLEGDPVVPASAQPDPPNPVQDS | 829.8 | 2486.3 | 83 |
| MRPL19 | |||
| FLSPEFIPPR | 602.4 | 1202.8 | 79 |
| ILHIPEFYVGSILR | 829.4 | 1656.8 | 68 |
| LDDSLLYLR | 555.2 | 1108.4 | 87 |
| DALPEYSTFDMNMKPVAQEPSR | 843.5 | 2527.3 | 80 |
| WSQPWLEFDoMMR | 822.1 | 1642.2 | 62 |
| IEAAIWNEIEASK | 737.7 | 1473.5 | 91 |