FIGURE 6.

The C locus controlled the Cameo2 expression in a tissue-specific manner likely by a cis-regulatory manner. A, schematic diagram of the principle of SNP analysis in F1 to elucidate whether Cameo2 expression is controlled in a cis-regulatory or trans-acting manner. B, SNP analysis in Cameo1 and Cameo2 of the C, +^C, and F1 larvae. mRNA and genomic DNA were from the middle and posterior silk glands, respectively. The stage was V2–V4. The SNP sites are indicated in supplemental Fig. S2. Similar SNP patterns were obtained from three individuals of F1 larvae. C and D, examination of tissue distribution of Cameo2 by Northern blotting (C) and RT-PCR (D) analyses. rpL3 is an internal control (28). The stage was W0 unless otherwise noted. E and F, comparison of carotenoid pigmentation in the silk gland, testis, and ovary between the C and +^C allele strains. Stages are indicated on the figures. White around the testis or ovary were fat body. Scale bar, 1 cm.