FIGURE 3.

Co-expression of WT PrP suppresses drug hypersensitivity induced by ΔCR PrP. A, HEK cells expressing ΔCR or WT PrP were mock-transduced, or transduced with a recombinant lentivirus encoding WT PrP. After 18 h, cells were incubated with Zeocin (500 μg/ml) for 2 days and then assayed for viability by MTT reduction. Cell viability is expressed as the A₅₇₀ value of drug-treated cells as a percentage of the value for untreated cells. B, HEK cells expressing ΔCR or WT PrP were mock-transduced or transduced with a recombinant lentivirus encoding WT PrP. After 3 days, cell lysates were prepared, and proteins were enzymatically deglycosylated with PNGase F, followed by Western blotting for PrP using 6D11 antibody. Bands corresponding to WT and ΔCR PrP are indicated by black and white arrowheads, respectively. Molecular size markers are given in kDa.