FIGURE 8.

Antibodies to the 93–104 region of ΔCR PrP inhibit drug sensitization. A, HEK cells expressing ΔCR PrP were pretreated with antibodies (5 μg/ml) for 60 min, after which Zeocin was added at a final concentration of 500 μg/ml. Forty-eight hours later, cells were assayed for viability by MTT reduction. Cell death (1 − A₅₇₀) is plotted as a percentage of the value for non-drug-treated cells. B, HEK cells expressing ΔCR PrP were pretreated with antibodies for 60 min, after which Zeocin was added to a final concentration of 500 μg/ml. Sixty minutes later, cells were lysed and analyzed for γ-H2AX as described in the legend to supplemental Fig. S5B. The bars show means for duplicate wells.