TABLE 1.

Drug sensitivity of HEK cells expressing WT or ΔCR PrP

Stably transfected HEK cells expressing WT or ΔCR PrP from a vector (pcDNA3.1(+) Hygro) encoding hygromycin resistance were treated with drugs at the indicated concentrations for 3 days (unless indicated otherwise) and then assayed for viability by MTT reduction. A positive toxic effect was defined as a reduction in viability of >50% in drug-treated cells compared with untreated cells. G418, hygromycin, Zeocin, and bleomycin (all shown in red) were selectively toxic to ΔCR but not WT cells under the indicated conditions. Other drugs had no effect on either cell type or were toxic to both. Vector-transfected cells behaved identically to cells expressing WT PrP in these assays (not shown).