FIGURE 10.
Spontaneous stepwise-stacking of proteins on ZnO via 4F2 VHH. A, reflectometric interference sensorgrams for interaction of 4F2 VHH (red, solid line), VHHZnOBP1 (blue, solid line), VHHZnOBPtag (blue, dotted line), and cAbBCII10 VHH (black, solid line) with ZnO film. Each VHH solution (1 μm) was injected for 240 s. B, reflectometric interference sensorgram (red, solid line) of the interaction of bispecific VHH dimers with ZnO film and GFP. VHH dimer solution (1 μm) was injected for 240 s, after which time GFP proteins were injected. Arrow indicates the timing of GFP injection. Black line corresponds to the sensorgram measured when only GFP proteins were injected for 240 s.