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A, size-exclusion chromatography for cAbBCII10 VHH, VHHZnOBP1, VHHZnOBP2, and VHHZnOBP3. Each 250-μl sample was applied to a Superdex 75 10/300 GL column, and the absorbance of the eluant was monitored at a wavelength of 280 nm. B, circular dichroism spectra for the ZnOBP-grafted VHH fragments of cAbBCII10 VHH, VHHZnOBP1, VHHZnOBP2, and VHHZnOBP3.
