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Frequencies of amino acids in randomized CDR 3 sequences of VHHZnOBP1 before selection against ZnO (A) and after the fourth selection procedure with ZnO particles (B) are shown. The ratios of selected amino acids that are obtained by dividing the values in B by those in A are shown in C. Color bars are amino acids as follows: His (blue), Arg (green), Leu (white), Gly (red), and Val (yellow).
