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SDS-PAGE (18% acrylamide) results of competitive inhibition assay for the binding of VHHZnOBPtag (A), VHHZnOBP1 (B), VHHsZnOBP3 (C), and 4F2 VHH for ZnO particles with the peptides of ZnOBP and sZnOBP (D). Lanes 1–8 correspond to added VHH solution, unadsorbed fraction, wash fraction, fractions eluted with 3, 30, and 300 μm, and 3 mm peptide solutions, and fraction eluted with 6 m GdnHCl, respectively.
