TABLE 4.

Thermodynamic parameters of Gibbs free energy (ΔG), enthalpy (ΔH), and entropy (ΔS) of the binding of 4F2 VHH, VHH_ZnOBP1, VHH_sZnOBP3, VHH_ZnOBPtag for ZnO surface

The values were calculated from the K_D values by van't Hoff equation.

Fragment	ΔH	TΔS	−ΔG
	kJ/mol	kJ/mol	kJ/mol
VHHZnOBPtag	−32.6	−3.6	36.2
VHHZnOBP1	−37.1	2.3	34.8
VHHsZnOBP3	−37.3	−0.5	37.8
4F2 VHH	−45.7	−1.8	43.9