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Selected VHH fragments after four rounds of selection against Al2O3 and CoO
Sequences selected by phage display methods are underlined. Numbering of the amino acids of VHHAlOBP1 and VHHCoOBP1 follows the Kabat numbering system (20). Amino acids of selected VHH are numbered as 3–1 to 3–16.
a KD is equilibrium constant.
b W is maximum adsorption amount.
c The amounts of 4H12 VHH fragments prepared by E. coli expression were too small to measure adsorption isotherms.
