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. 2010 Jan 6;285(10):7827–7837. doi: 10.1074/jbc.M109.040618

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Overexpression of TRIM5α induces a morphological change in HEK293T cells. A, the effect of TRIM5α overexpression was evaluated in HEK293T cells transfected with HA-tagged human (Hu), rhesus monkey (Rh), or African green monkey (AGM) TRIM5α or an empty vector pcDNA3.1(−) as a control. Morphological changes were observed under microscope at 48 h post-transfection. B, the effect of TRIM5α overexpression was evaluated in HEK293T cells transfected with GFP-tagged rhesus TRIM5α or pEGFP-C1 as a control. Phase contrast (left panels) and GFP fluorescence (right panels) were analyzed at 48 h post-transfection. The arrows in the phase-contrast image indicate the morphological change (retraction of cellular borders and rounding of cells) induced by TRIM5α overexpression. C, HEK293T cells were transfected with HA-tagged rhesus TRIM5α, AGM TRIM5α, AGM TRIM5α containing the PRYSPRY domain of rhesus TRIM5α, or rhesus TRIM5α containing the PRYSPRY domain of AGM TRIM5α for 48 h and analyzed by phase-contrast microscopy.