FIGURE 3.

The PRYSPRY domain of TRIM5α is required for Hsp70 binding. A, schematic representation of wild-type (WT) rhesus TRIM5α and TRIM5α variants with deletion of the RING domain, B-box domain, CC domain, or PRYSPRY domain is shown. B, co-immunoprecipitation of FLAG-Hsp70 with HA-tagged rhesus TRIM5α variants. HEK293T cells were transfected with FLAG-Hsp70 and HA-tagged wild-type TRIM5α or TRIM5α variants containing the deletion of each domain, and the cell lysates were subjected to immunoprecipitation and Western blotting as described in Fig. 2.