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Co-expression of Hsp70 reverses TRIM5α-induced morphological change. HEK293T cells were transfected with HA-tagged rhesus TRIM5α, FLAG-tagged Hsp, or both. Forty-eight hours after transfection, phase-contrast images were obtained. Cells were transfected with: an empty vector pcDNA3.1 (A), FLAG-Hsp70 (B), FLAG-Hsc70 (C), HA-rhesus TRIM5α (D), HA-rhesus TRIM5α and FLAG-Hsp70 (E), and HA-rhesus TRIM5α and FLAG-Hsc70 (F).
