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The ATPase function of Hsp70 is critical for reversing the TRIM5α-induced morphological change. HEK293T cells were transfected with: an empty vector pcDNA3.1 (A), FLAG-Hsp70 (B), HA-rhesus TRIM5α (C), HA-rhesus TRIM5α and FLAG-WT-Hsp70 (D), HA-rhesus TRIM5α and FLAG-K71E-Hsp70 (E), and HA-rhesus TRIM5α and FLAG-K71M-Hsp70 (F). Forty-eight hours after transfection, phase-contrast images were obtained.
