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. 2010 Jan 6;285(10):7827–7837. doi: 10.1074/jbc.M109.040618

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FIGURE 8. — Co-expression of Hsp70 elevates the level of TRIM5α in the detergent-soluble fraction. A, HEK293T cells were transfected with HA-tagged rhesus TRIM5α and increasing amounts of FLAG-Hsp70. Forty-eight hours after transfection, the cell lysates were separated into the detergent-soluble and detergent-insoluble fractions by centrifugation and evaluated for HA, FLAG, and β-actin by Western blotting. B, HEK293T cells were transfected with FLAG-Hsp70, HA-tagged rhesus TRIM5α, or both. Forth-eight hours after transfection, cells were fixed and labeled with HA antibody (green) and/or FLAG antibody (red). The arrows in the phase-contrast images indicate the morphological change (retraction of cellular borders and rounding of cells) induced by TRIM5α overexpression.