TABLE 1.

The oligonucleotide primers used to generate the variants of TRIM5α and Hsp70

Primer	Nucleotide sequence (5′ to 3′)
pcDNA3.1-for	AAGCAGAGCTCTCTGGCTAAC
pcDNA3.1-rev	TCCAGGGTCAAGGAAGGCACG
RING-for	ACTGATCCGGGTCACCTCCTCCTTTACATTAAG
RING-rev	GGTGACCCGGATCAGTTACCAGCCTGAG
B-box-for	GGAAAGTGTGATCAACCTTCTGTCCCTC
B-box-rev	TTGATCACACTTTCCTCATGGAGGAGGTTG
CC-for	CTGAGATGAAAGTGTGGTGACCACGGTGC
CC-rev	CACTTTCATCTCAGAACTGGAGCATCG
PRYSPRY-fora	GAGAGCTAACAGATGCCTGAATTCGCTACTGGGTTGATGTGAC
PRYSPRY-rev	GTCACATCAACCCAGTAGCGAATTCAGGCATCTGTTAGCTCTC
Hsp70-for	GCATGCGGCCGCGATGGCCAAAGCCGCGGC
Hsp70-rev	GACTTCTAGACTAATCTACCTCCTCAATGGTGGGGC
Hsc70-for	GCATGCGGCCGCGATGTCCAAGGGACCTGCAGTTGG
Hsc70-rev	GACTTCTAGATTAATCAACCTCTTCAATGGTGGGCC
K71E-for	CGCAGAACACCGTGTTTGACGCGGAGCGGCTGATCGGCCGCAAGTTCGG
K71E-rev	CCGAACTTGCGGCCGATCAGCCGCTCCGCGTCAAACACGGTGTTCTGCG
K71M-for	CCGCAGAACACCGTGTTTGACGCGATGCGGCTGATCGGCCGCAAGTTCGG
K71M-rev	CCGAACTTGCGGCCGATCAGCCGCATCGCGTCAAACACGGTGTTCTGCGG

^a The stop codon (bold case) and EcoRI restriction site (italic) introduced are indicated.