Fig. 3.

Expression of the >105 kDa gpUL18 form was enhanced by β₂m. (a) Immunoblot of cell lysates prepared from HFFFs infected with v-UL18, or co-infected with v-UL18 and v-β₂m as indicated. Cell lysates were divided into three equal parts and either left mock treated (–), or digested with EndoH (+E) or PNGaseF (+P). gpUL18 was detected using the mAb M71. (b) HFFFs were infected with v-UL18 or v-Ctrl alone, or co-infected with v-UL18 and v-β₂m as indicated. Immunoprecipitation was performed using the mAb M71, one half of each sample was then treated with EndoH.