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. Author manuscript; available in PMC: 2010 Mar 23.
Published in final edited form as: Nat Immunol. 2005 Jan 9;6(2):181–188. doi: 10.1038/ni1156

Figure 5.

Figure 5

UL141-mediated downregulation of cell surface CD155 detected by fluorescence microscopy. CD155 expression monitored by staining with the monoclonal antibodies D171 or 5D1 in the presence or absence of UL141. The gpUL141 was expressed with an adenovirus vector (RAdUL141-GFP), infection with strain Toledo-GFP in HFFFs or in UL141-GFP–transfected HEK 293 cells. Similarly, gpUL16 was expressed with an adenovirus vector (RAdUL16-GFP) or in UL16-GFP-transfected cells. HCMV strain AD169 is UL141 negative. HCMV and adenovirus vectors contain an intact GFP gene to identify infected cells. In HEK 293 cells, gpUL16-GFP and gpUL141-GFP fusion proteins traffick to the endoplasmic reticulum and concentrate in intracytoplasmic foci.