Fig. 2.

FRG-1 and FRG1 proteins bind and bundle F-actin. (A) High-speed F-actin cosedimentation assays were performed at varying concentrations of FRG-1 with a constant concentration of F-actin. (B) Western blot analysis of a representative glutaraldehyde crosslinking time course using recombinant FRG-1. Monomer (30 kDa), dimer (60 kDa) and tetramer (120 kDa) species were present. (C-F) Electron micrographs of actin bundles formed with recombinant (C,E) C. elegans FRG-1 or (D,F) human FRG1. (C,D) Scale bars: 500 nm. (E,F) Scale bars: 200 nm.