Fig. 5.
Overexpressed FRG-1 preferentially localizes to the nucleus and shows neomorphic localization. (A) Pmyo-3::frg-1::gfp transgenic animals express FRG-1::GFP strongly in body-wall muscle nuclei and weakly in the dense bodies. Animals transgenic for Pfrg-1::frg-1::gfp show visually low (B,D) and high (C,E) levels of FRG-1::GFP expression in body-wall muscle cells (B and C) and nuclei (D and E). High levels of FRG-1 expression lead to coating of the F-actin filaments (compare C, FRG-1-stained striations with B, punctate dense-body staining) and nuclear aggregate formation (E, arrowheads, compared with D) in addition to normal nucleolar staining (arrows). Scale bars: 10 μm.