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. 2010 Mar 23;123(7):1124–1130. doi: 10.1242/jcs.060178

Fig. 1.

Fig. 1.

MRG15 associates with PALB2 in a protein complex. (A) Immunopurified FLAG-MRG15- or FLAG-MRGX-containing complexes resolved by SDS-PAGE and silver stained. A mock purification from cells containing the empty expression vector, with the FLAG epitope tag, is shown as a control. Proteins identified by LC-MS/MS analysis are indicated on the right. (B) An immunopurified FLAG-HA-PALB2-containing complex resolved and analyzed as in A. (C) Whole-cell lysates (Input) of HeLa cells expressing FLAG-MRG15 (top) or FLAG-PALB2 (bottom) and fractions immunoprecipitated (IP) using anti-FLAG M2 beads subjected to western blotting using the antibodies indicated. (D) Whole-cell lysates (Input) of HEK293T cells expressing FLAG-MRG15 and fractions immunoprecipitated (IP) using anti-FLAG M2 beads subjected to immunoblotting using the antibodies indicated. (E) Each purified GST fusion protein was examined for its ability to bind 6×His-PALB2. Input, non-bound (FT), and bound fractions were analyzed by western blotting with anti-6×His or anti-GST antibodies.