Fig. 2.

MRG15 stimulates homologous recombination. (A) U2OS cells were treated with control or indicated siRNAs and the levels of endogenous MRG15, PALB2, BRCA2, MRGX or α-tubulin (loading control) were analyzed by western blotting. (B) Relative levels of BRCA2 mRNA were analyzed in U2OS cells following a control transfection or transfection with cocktails of siRNAs directed against BRCA2 or MRG15. (C) U2OS-DR cells were treated with control or indicated cocktails of siRNAs and assayed for HR. Representative data obtained by FACS analysis are shown with average levels of GFP-positive cells indicated. (D) Relative HR ratios (% ± s.d.) calculated from three independent experiments. Cocktails of siRNAs directed against MRG15, MRGX, PALB2 or BRCA2 were used.