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. 2010 Mar 23;123(7):1124–1130. doi: 10.1242/jcs.060178

Fig. 5.

Fig. 5.

MRG15 is involved in the localization of PALB2, BRCA2 and RAD51, but not BRCA1, to DNA-damage foci. (A-D) U2OS cells transfected with siGFP or siMRG15-3′-UTR#1 were fixed 16 hours after exposure to 8 Gy IR or were left untreated. Cells were labeled with anti-PALB2 or γ-H2AX antibodies and counterstained with DAPI to visualize nuclei. Examples of colocalization in merged images are shown (A), along with quantification of the percentage of cells (mean ± s.d. of three counts) with five or more PALB2 foci (B), the percentage of cells with five or more γ-H2AX foci (C), or in which PALB2 foci were detected in γ-H2AX-positive cells (D). (E) The percentage of cells transfected with siGFP, siMRG15-3′-UTR#1, or siMRG15-3′-UTR#2 with five or more PALB2, BRCA2, RAD51 or BRCA1 foci 16 hours after exposure to 8 Gy IR.