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. 2010 Mar 17;17(1):18. doi: 10.1186/1423-0127-17-18

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Copyright ©2010 Ro et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of a Gsk3β inhibitor on gene expression in PC12 (DN-Akt) cells. PC12 (DN-Akt) cells were treated with 10 μM TWS119, a Gsk3β inhibitor for three hours. (A) Western blot analysis of β-catenin in PC12 (DN-Akt) cells treated with TWS119 to confirm that this agent works actively as a Gsk3β inhibitor in our experimental conditions. (B) mRNA levels for each gene are presented as a fold difference over that of vehicle-treated cells. *P < 0.05 (n = 3-4, mean + SD). (C) Western blot analysis of Syn-1 protein. Veh, vehicle; Inh, inhibitor.