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shRNA expression vector against Syn-1 reduces the endogenous Syn-1 transcript and protein levels. PC12 (DN-Akt) cells were infected with lentiviral shSyn-1#1, #2 or shControl (shCtrl). Syn-1 transcript was detected by semi-quantitative (A) and real-time (B) RT-PCR. (C) Western blot analysis.
