Table 1.
Primer Information.
| Primer Name | Primer Sequence | Tm (°C) * |
|---|---|---|
| Vector Primers | ||
| M13F | 5'-GTAAAACGACGGCCAG-3' | 50.7 |
| M13R | 5'-CAGGAAACAGCTATGAC-3' | 47.0 |
| T3 | 5'-ATTAACCCTCACTAAAGGGA-3' | 50.3 |
| T7 | 5'-TAATACGACTCACTATAGGG-3' | 47.5 |
| Gene Primers | ||
| 14321 5' F1 | 5'-AGGGTTAGGTTTAAGGTTGAG-3' | 51.8 |
| 14321 5' F2 | 5'-GTGAGGGTACGAAAGTAGG-3' | 51.9 |
| 14321 3' F1 | 5'-TCGCAAGTTGAACTATGTATCC-3' | 52.6 |
| 14321 3' F2 | 5'-CAGTTTGTTCAGGACTGAGT-3' | 52.2 |
Vector primer sequences are standard, and are provided here for convenience. Gene primers were designed by us.
*Melting temperature values were determined by the Integrated DNA Technologies OligoAnalyzer application [7], and may not be the same as those published elsewhere for M13_ and T_ primers.