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Cell Cycle associated H2AX phosphorylation in ATM deficient cells. The levels of phosphorylated H2AX at different times after release from G1 block were detected in ATM deficient cell lines ATS4 (A) and AT5BIVA (B). Synchronized G1 cells were cultured in fresh DMEM, harvested at 0, 5, 8 and 10 h after cell cycle re-entry, and protein expression was assayed by Western blotting.
