Table 1.
Endpoint and real-time PCR primers employed in this study
| Primer | Sequence (5'-3') | Position | Target gene | Reference |
|---|---|---|---|---|
| Endpoint PCR | ||||
| A189 amoA2R' |
GGHGACTGGGAYTTCTGG CCTCKGSAAAGCCTTCTTC |
151-168 802-820 |
amoA | [36,37] |
| HAO1F HAO1R |
TCAACATAGGCACGGTTCATCGGA ATTTGCCGAACGTGAATCGGAACG |
203-226 1082-1105 |
hao | [38] |
| NirK1F NirK1R |
TGCTTCCGGATCAGCGTCATTAGT AGTTGAAACCGATGTGGCCTACGA |
31-54 809-832 |
nirK | [38] |
| NorB1F NorB1R |
CGGCACTGATGTTCCTGTTTGCTT AGCAACCGCATCCAGTAGAACAGA |
479-502 1215-1238 |
norB | [38] |
| KNO50F KNO51R |
TNANACATGCAAGTCGAICG GGYTACCTTGTTACGACTT |
49-68 1492-1510 |
Eubacterial 16S rRNA gene | [39] |
| Quantitative PCR | ||||
| amoAFq amoARq |
GGACTTCACGCTGTATCTG GTGCCTTCTACAACGATTGG |
408-426 524-543 |
amoA | [15] |
| HAO1Fq HAO1Rq |
TGAGCCAGTCCAACGTGCAT AAGGCAACAACCCTGCCTCA |
266-285 331-350 |
hao | [38] |
| NirK1Fq NirK1Rq |
TGCAGGGCATACTGGACGTT AGGTGAACGGGTGCGCATTT |
182-201 291-310 |
nirK | [38] |
| NorB1Fq NorB1Rq |
ACACAAATCACTGCCGCCCA TGCAGTACACCGGCAAAGGT |
958-977 1138-1157 |
norB | [38] |
| EUBF EUBR |
TCCTACGGGAGGCAGCAGT GGACTACCAGGGTATCTAATCCTGTT |
339-357 780-805 |
Eubacterial 16S rRNA gene | [34] |