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. 2010 Mar 24;5(3):e9846. doi: 10.1371/journal.pone.0009846

Figure 4. Induction of copy-number variation.

Figure 4

A, Flow cytometric profiles of double-targeted cells, Cre-activated cells, and Cre-terminated cells kept OHT-free for 10 days (top). Flow cytometric profiles of sorted cell clones and a DsRed-positive clone 10 days after Cre activation (bottom). B, PCR confirmation of rearrangement for clones from cis- and trans-targeting cells. Clone numbers from 1 to 5 correspond to rearranged clone numbers (post) in A. Clones 6 to 9 are derived from trans-targeted cells. Cells before Cre activation (pre), Nalm-6 and the no-template control (H2O) are included. Triangles above the transgene schemes represent primer positions (IgA2-B41-F1 and 104-B41-R1 for green, and 104-B23-F1 and IgA2-B23-R1 for red).