Table 1. Targeting configuration of NSK cells with loxP sites spanning 1.1 Mb in the same direction.

Targeting configuration	Clone	No. of metaphase	Colocalization	Non-colocalization	Frequency of fluorescence-positive cells
cis	NSK122	8	7	1	1.2×10−2
	NSK126	7	7	0	1.3×10−2
	NSK164	7	7	0	1.6×10−2
	NSK171	8	7	1	2.0×10−2
	mean frequency (mean ± standard deviation)				1.5±0.4×10−2
trans	NSK107	7	0	7	3.7×10−4
	NSK108	12	1	11	3.0×10−4
	NSK114	7	0	7	3.6×10−4
	NSK116	16	2	14	1.8×10−4
	NSK123	8	1	7	2.5×10−4
	NSK145	8	1	7	2.0×10−4
	mean frequency of (mean ± standard deviation)				2.8±0.8×10−4
mixed	NSK72	7	3	4	3.8×10−3
	NSK141	14	6	8	2.3×10−4

The numbers of metaphases analyzed and those which exhibited colocalization or non-colocalization of signals by Site1 and Site2 probes are shown with frequencies of double-positive cells after 7 days of OHT stimulation.