Table 2. Targeting configuration of NSC cells with loxP sites spanning 1.1 Mb in the opposite direction.
Targeting configuration | Clone | No. of metaphase | Colocalization | Non-colocalization | Frequency of fluorescence-positive cells |
cis | NSC27 | 8 | 8 | 0 | 1.0×10−2 |
NSC71 | 8 | 8 | 0 | 8.2×10−3 | |
NSC130 | 8 | 7 | 1 | 9.6×10−3 | |
mean frequency (mean ± standard deviation) | 9.3±0.9×10−3 | ||||
trans | NSC94 | 16 | 1 | 15 | 8.8×10−5 |
NSC169 | 8 | 0 | 8 | 7.7×10−5 | |
mean frequency (mean) | 8.3×10−5 |
The numbers of metaphases analyzed and those which exhibited colocalization or non-colocalization of signals by Site1 and Site2 probes are shown with frequencies of double-positive cells after 7 days of OHT stimulation.