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. 2010 Mar 24;5(3):e9853. doi: 10.1371/journal.pone.0009853

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Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Shown is the promoter region and luciferase cDNA. The upstream primer started at −324 bp and the downstream primer started at +23 bp from transcriptional start site of luciferase gene. Primers denoted by arrows. CRE-cyclic AMP response element; TATA-TATA box.