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. Author manuscript; available in PMC: 2010 Mar 23.
Published in final edited form as: J Orthop Res. 2005 Jun 4;23(6):1300–1307. doi: 10.1016/j.orthres.2005.04.003.1100230610

Fig. 1. Timing of chondrocyte differentiation and maturation is age-dependent.

Fig. 1

(A) By day 3, the periosteum (bracket) adjacent to the fracture site had thickened in juvenile mice. A small locus of cells (dotted line) in the periosteum was stained by safranin-O, (B) and they were expressing ColII (red). In (C) middle-aged and (E) elderly mice, the periosteum (brackets) was slightly thickened, but (D, F) no ColII expression was evident. (G) By day 5, juvenile mice exhibited evidence of cartilage matrix production (light red) in the fracture callus, and (H) robust ColII expression was evident. (I) ColX expression (yellow) was detected in the ColII domain. In (J, K) middle-aged and (M, N) elderly mice chondrocytes stained by SO/FG and expressing ColII were evident at day 5, but (L, O) no ColX expression was observed. H, K, N (ColII) and I, L, O (ColX) are high magnifications of areas boxed in G, J, M. SO/FG=safranin-O/fast green staining. Scale bar A-F, H, I, K, L, N, O= 200 µm, G, J, M = 1mm.