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. Author manuscript; available in PMC: 2011 May 10.
Published in final edited form as: Virology. 2010 Feb 25;400(2):224–232. doi: 10.1016/j.virol.2010.02.001

Fig. 3.

Fig. 3

Profile of CD8+ T cells specific for immunodominant NP366–374 (NP) and PA224–233 (PA) epitopes at day 8 post-infection in selected tissue compartments. MLK3−/− and wild-type (WT) mice were intranasally infected with 400 EID of PR8. A) Lungs were isolated at day 8 post-infection and processed and assayed by flow cytometry for the presence of NP and PA-specific CD8+ T cells. Data points represent individual mice with 4–5 mice per group; bars denote mean for the group. Results shown are representative of 3 individual experiments. B) ELISPOT analysis of influenza virus-specific CD4 and CD8 IFN-γ producing cells. Spleen and mediastinal lymph node (MLN) were isolated at days 4, 6, 8 and 10 after infection. Cells from the spleen (pooled) and MLN (pooled) were purified using MACS separation. The number of total IFN-γ secreting cells was quantified using ELISPOT analysis with peptides for 2 immunodominant epitopes for CD4 and CD8. Depicted is the average number of spots from triplicate wells. Spot forming units is abbreviated as s.f.u. Results shown are representative of two separate experiments.