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. Author manuscript; available in PMC: 2011 May 14.
Published in final edited form as: Mol Cell Endocrinol. 2010 Feb 17;320(1-2):76–86. doi: 10.1016/j.mce.2010.02.014

Figure 3.

Figure 3

GR resistance is reversible. A) The 697-shGR2-F5 clone was pre-treated with Doxy for 48 hours and subsequently treated with 1 μM Dex or an equal volume of EtOH. After 72 hours of treatment, the Dex-treated cells were washed with PBS (4–5 times over 72 hours) and cultured in fresh media without Doxy. B) After 72 hours RFP expression had nearly ceased and the washed and re-cultured cells were re-treated with either 1 μM Dex or an equal volume of EtOH. Assays were quantitated via Trypan Blue exclusion and hemacytometry.