FIG. 6.

Amino acid transport characteristics in cumulus-enclosed GV oocytes. The rates of transport of each radiolabeled amino acid as indicated by the labels in A–I were measured for GV oocytes that had been within intact COCs during incubation with radiolabeled amino acids for 10 min. Cumulus cells were removed before measurement of radiolabeled amino acid content. The black bars indicate the rate of uptake in the absence of any addition, and potential competitive inhibitors used (10 mM each) are indicated by labels under the gray bars at the bottom of each panel. Each bar indicates the mean ± SEM of at least three independent measurements. Bars not sharing the same letter within a given stage are significantly different (P < 0.05 by ANOVA with Tukey-Kramer post hoc test). NS, no significant difference.