Skip to main content
. 2009 Sep 16;82(1):123–131. doi: 10.1095/biolreprod.109.076588

FIG. 2.

FIG. 2.

Polyadenylation of Slbp mRNA during maturation. A) Oocytes were collected at the indicated stages of maturation, and the length of the poly(A) tails of Slbp and Ccnb1 (cyclin B1) were analyzed using a PCR-based assay. The PCR product comprises the portion of the mRNA that lies 3′ of the gene-specific primer, the poly(A) tail, and the ligated short RNA. The length of the poly(A) tail was determined by sequencing cloned products. B and C) Oocytes were treated with DMSO or epoxomycin for the indicated period of times. Arrows in C indicate the two products detected for Slbp.