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. 2009 Sep 16;82(1):123–131. doi: 10.1095/biolreprod.109.076588

FIG. 4.

FIG. 4.

Effect of proteasomal inhibition on the accumulation of SLBP during maturation. A) Immature oocytes were allowed to begin maturation, then, after 3 h (left), 8 h (middle), or 12 h (right), were transferred to medium containing no drug, LLM, or MG132, and incubated for an additional 12–15 h. Samples were also taken before the drug treatment for experiments shown in the middle and right panels. Samples were immunoblotted using antibodies recognizing SLBP and MAPK1/3 (loading control); 50 oocytes per lane. B) Results of a separate series of three experiments that were analyzed quantitatively. Oocytes were transferred to MG132 or DMSO after 3 or 8 h of maturation, as indicated. Mean values were compared using the t-test. C) Maturing oocytes were transferred to medium containing DMSO or epoxomicin at the indicated times and collected at 20 h. Results of four experiments are shown; 30 oocytes per group. Error bars indicate the standard error of the mean.