Figure 4.

Effects of HRHs on PRL secretion as quantified by reverse hemolytic plaque assay. Cells were incubated concurrently with PRL antibody and the stated HRHs (100 nM) for 60 min. (see Materials and Methods). (A) Effects of HRHs on the percentage of plaque-forming cells. Data are the mean ± SEM of five independent experiments. (B) Inhibition of the LHRH effect by the antagonist [d-Phe2,6,Pro3]-LHRH (+A) at 300 nM; +SOM, effects of somatostatin at 100 nM. Data are representative of two similar experiments. Differences were considered significant (∗) at P < 0.05.