Fig. 5.
Validation of the docking structure. a Intermolecular NOEs observed for 15N/2H-labeled LBT-DR complexed with unlabeled KE. The atoms between which NOEs were observed are displayed in a stick model and connected by dotted lines. The structure is shown in the direction rotated by 30°, along the longitudinal axis, from that in Fig. 4a and b. b and c Comparison of experimental and back-calculated PCSs of backbone amide protons observed for LBT-DR/KE in complex with Tb3+ (b) and Tm3+ (c). The tensors were calculated using the whole lowest energy structure of the LBT-DR/KE complex