Table 1.
Method | Virus | Target | Primers | Sequence (5′–3′) | Amplicon size |
---|---|---|---|---|---|
Real-time RT-PCR | Norovirus GI | ORF1-ORF2 conjunction | GI-F | CGYTGGATGCGNTTYCATGA | 85 |
GI-R | CTTAGACGCCATCATCATTYAC | ||||
Norovirus GII | ORF1-ORF2 conjunction | GII-F | CARGARBCNATGTTYAGRTGGATGAG | 98 | |
GII-R | TCGACGCCATCTTCATTCACA | ||||
Multiple RT-PCR | Norovirus GI | Capsid | G1-SKF | CTGCCCGAATTYGTAAATGA | 330 |
GI-SKR | CCAACCCARCCATTRTACA | ||||
Norovirus GII | Capsid | COG2F | CARGARBCNATGTTYAGRTGGATGAG | 387 | |
G2-SKR | CCRCCNGCATRHCCRTTRTACAT | ||||
Astrovirus | Capsid | Mon269 | CAACTCAGGAAACAGGGTGT | 449 | |
Mon270 | TCAGATGCATTGTCATTGGT | ||||
Sapovirus | Capsid | SLV-5317 | CTCGCCACCTACRAWGCBTGGTT | 434 | |
SLV-5749 | CGGRCYTCAAAVSTACCBCCCCA |
RT-PCR = reverse transcriptase-polymerase chain reaction; ORF = open reading frames.